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SRX2658024: GSM2544757: T0G3; [Enterobacter] lignolyticus SCF1; RNA-Seq
6 ABI_SOLID (AB 5500xl Genetic Analyzer) runs: 24.2M spots, 1.2G bases, 949.5Mb downloads

Submitted by: NCBI (GEO)
Study: Multi-time series RNA-Seq analysis of Enterobacter lignolyticus SCF1during growth in lignin-amended medium
show Abstracthide Abstract
The production of lignocellulosic-derived biofuels is a highly promising source of alternative energy, but it has been constrained by the lack of a microbial platform capable to efficiently degrade this recalcitrant material and cope with by-products that can be toxic to cells. Species that naturally grow in environments where carbon is mainly available as lignin are promising for finding new ways of removing the lignin that protects cellulose for improved conversion of lignin to fuel precursors. Overall design: Genome-wide transcriptomic analysis of cultures of SCF-1 were sampled along three times during batch anaerobic batch growth in lignin amended medium versus control (unamended medium).
Sample: T0G3
SAMN06622154 • SRS2061128 • All experiments • All runs
Library:
Instrument: AB 5500xl Genetic Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted using Trizol following by DNAseI treatment using Qiagen RNAseay mini column Template cDNA was prepared using the Applied Biosystems SOLiD® Total RNA-Seq Kit (Life Technologies, Carlsbad, CA) according to manufacturer's protocol.
Experiment attributes:
GEO Accession: GSM2544757
Links:
Runs: 6 runs, 24.2M spots, 1.2G bases, 949.5Mb
Run# of Spots# of BasesSizePublished
SRR53623134,561,006228.1M183.1Mb2018-02-14
SRR53623143,994,881199.7M159.6Mb2018-02-14
SRR53623154,207,488210.4M166.5Mb2018-02-14
SRR53623163,698,733184.9M145.5Mb2018-02-14
SRR53623174,030,413201.5M154.9Mb2018-02-14
SRR53623183,663,567183.2M139.9Mb2018-02-14

ID:
3841366

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